Learn about Serological Testing by completing the following lab simulation.
Objectives:
- To define the following terms: antigen, antibody, epitope, precipitin line, and serum.
- To distinguish between antigens and antibodies.
- To learn how the following serological tests are performed: direct fluorescent antibody technique, Ouchterlony technique (double diffusion), enzyme linked immunosorbent assay and Western blotting technique.
- To understand the specificity that antigens and antibodies have for each other.
- To relate non-specific binding to false positive results.
- To distinguish between positive and negative controls.
- To compare and contrast the ELISA and Western blotting technique.
