Lab Quiz

Serological Testing Lab Quiz

This activity contains 21 questions.

	Refer to Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia. Positive Controls are important for an experiment because   they give a standard against which the results can be compared. they give a range of acceptable results. they reduce the chance of false positives. they supply a standard to identify false negatives.

	Which patient(s) would be considered positive for Chlamydia?   Patient A is positive for Chlamydia. Patient B is positive for Chlamydia. Patient C is positive for Chlamydia. Patients A and B tested positive for Chlamydia. Patients B and C tested positive for Chlamydia. All of the patients tested positive for Chlamydia.

	Direct Fluorescent Antibody Technique detects the presence of what immune-related protein?   This technique tests for the presence of non-specific proteoglycans. This technique tests for the presence of antibodies. This technique tests for the presence of transport proteins. This technique tests for the presence of ligand binding proteins. This technique tests for the presence of antigens.

	The purpose of washing in the Direct Fluorescent Antibody Technique was to   dilute the antibody solution to reduce the concentration of fluorescent molecules. keep the slide from drying out. clean off the slide. rinse away non-specific binding.

	Refer to Activity 2: Ouchterlony Technique. Another name for the Ouchterlony Technique is   active transport. double diffusion. facilitated diffusion. carrier-mediated transport.

	The Ouchterlony Technique is designed to determine what?   It determines if antibodies are identical. It determines if the antigens are identical. It determines is the antibodies are unrelated. It determines is the epitopes are active. It determines if the antigens are contaminated.

	The precipitin line will appear under which of the following conditions?   The ratio of antigens to antibodies is 2 to 1. All antigens have become bound by antibodies. The ratio of antibodies to antigens is 2 to 1. The antibody-antigen concentrations are in optimal proportions.

	What is the identity of the unknown in the Ouchterlony Technique activity?   It is unrelated to BSA. It has identity with HSA. It has partial identity with BSA. It has partial identity to HSA. It has identity to BSA. It is unrelated to HSA.

	Which components are the antigens in the Ouchterlony Technique activity?   The HSA is an antigen. The agarose is an antigen. The goat A-H is an antigen. The goat A-B is an antigen.

	Refer to Activity 3: Using Indirect ELISA to Test for HIV. Which patient(s) would be considered positive for HIV?   Patient A tested positive for HIV. Patient B tested positive for HIV. Patient C tested positive for HIV. All of the above patients tested positive for HIV.

	The presence of what is being tested for in the patients' samples?   The presence of antigens is being tested. The presence of antibodies is being tested. The presence of substrate is being tested.

	What is the key substance in the Developing Buffer in the ELISA test?   Primary antibodies are the key substance of the Developing Buffer. Antigens are the key substance of the Developing Buffer. Reactive enzymes are the key component of the Developing Buffer. Secondary antibodies conjugated to the enzyme are the key component in the Developing Buffer.

	What type of ELISA test would this experiment be considered and why?   It is an indirect Elisa test because it is testing for antigens from the patients' samples. It is a direct Elisa test because it is testing for antibodies from the patients' samples. It is a direct Elisa test because it is testing for antigens from the patients' samples. It is an indirect Elisa test because it is testing for antibodies from the patients' samples.

	Which patient would be positive for seroconversion?   Patient A is positive for seroconversion. Patient B is positive for seroconversion. Patient C is positive for seroconversion. Patient D is positive for seroconversion. Patient E is positive for seroconversion.

	Refer to Activity 4: Using the Western Blot Strips to Detect Anti-HIV Antibodies. Which of the patients tested positive for HIV?   Patient A tested positive for HIV. Patient B tested positive for HIV. Patient C tested positive for HIV. None of the patients tested positive for HIV.

	In Activity 4, which of the following does NOT describe how the protein samples are separated?   The protein samples are separated in a liquid matrix. The protein samples are separated based on their charge. The protein samples are separated based on their size. The protein samples are separated using an electric current.

	Another term for Western blotting is   immunoblotting Southern analysis immunostaining Northern analysis immunoprecipitation

	In the Western Blot activity, what is transferred onto the nitrocellulose membranes?   Antigens are transferred onto the nitrocellulose membranes. Antibodies are transferred onto the nitrocellulose membranes. The detection substrate is transferred onto the nitrocellulose membrane.

	In the Western Blot activity, __________ is used to indicate the presence of the sample protein.   charge structural shape color size

	When testing for HIV using the Western Blot technique, an example of an indeterminate result would be:   negative results from the negative control. positive results for p33 and p24 only. no bands present. positive results from the positive control.

	Which test do you think would be more specific?   The Western Blot test would be more specific. The ELISA test would be more specific. They would both be equally specific.

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